Video: Restriction Enzyme Digests: Principle, Procedure, and Applications
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Video: Restriction Enzyme Digests: Principle, Procedure, and Applications
298.4K Views. Restriction enzymes or endonucleases recognize and cut DNA at a specific sequence. These enzymes occur naturally in bacteria as a defense against bacteriophages - viruses that infect bacteria. Bacterial restriction enzymes cut the invading bacteriophage DNA while leaving the bacterial genomic DNA unharmed due to addition of methyl ...
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When a typical restriction enzyme cuts a DNA molecule, the cuts... | Filo
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14K views · 446 reactions | EcoRI cutting a strand of DNA. EcoRI is a restriction endonuclease that recognizes and cuts DNA at a specific motif (GAATTC). After the DNA is cut, so-called sticky ends are left behind. Sticky ends are short non-paired DNA bases. This property makes this enzyme a powerful tool in molecular biology. The enzyme naturally occurs in E. coli, where it serves as a defense mechanism against viral DNA. | Ribosome Studio | Facebook
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14K views · 446 reactions | EcoRI cutting a strand of DNA. EcoRI is a restriction endonuclease that recognizes and cuts DNA at a specific motif (GAATTC). After the DNA is cut, so-called sticky ends are left behind. Sticky ends are short non-paired DNA bases. This property makes this enzyme a powerful tool in molecular biology. The enzyme naturally occurs in E. coli, where it serves as a defense mechanism against viral DNA. | Ribosome Studio | Facebook
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SOLVED: Restriction enzyme lab. The individual bands in the DNA ladder are visible, but only one band is observed in all the other wells (=multiple fragments are not observed in the other wells).NaCO3 was not added into the reaction.Lactase was not added into the reaction.Enzyme lab. The color will continuously increase in intensity until substrate enzymes are used up.Restriction buffer was left out of the restriction digest.The spectrophotometer was not calibrated to the solvent (water) prior t
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